OSSAPIG-NF-NF1(NS) MINIATURE SWINE (nf1 hindiii restriction fragment length polymorphism (rflp) positive for nf1-r1947x nf1- ns

Země: Spojené státy

Jazyk: angličtina

Zdroj: NLM (National Library of Medicine)

Aktivní složka:

SUS SCROFA (G.(NC_010454.4)43530860G>T + 43530872C>T + 43530892C>T + 43530899T>C) ONE COPY IN OSSABAW BREED MINIATURE SWINE (UNII: J64839N3EH) (SUS SCROFA (G.(NC_010454.4)43530860G>T + 43530872C>T + 43530892C>T + 43530899T>C) ONE COPY IN OSSABAW BREED MINIATURE SWINE - UNII:J64839N3EH)

Dostupné s:

Recombinetics, Inc.

Podání:

NOT APPLICABLE

Terapeutické indikace:

Limitations: This is a model of progressive disease. Some of the disease symptoms associated with Neurofibromatosis Type I disease in humans take time to develop, and like the human disease, are variable in presentation from animal to animal. Product Use: Model animals and/or materials derived from these animals are intended for purchase only under license agreement with Recombinetics, Inc., and solely for use in connection with the specific licensed biomedical research. Conditions of Use: This model animal and any products derived from it are for biomedical research purposes only and not for use in humans. This is an investigational animal. Edible products of investigational animals are not to be used for food unless authorization has been granted by the U.S. Food and Drug Administration or by the U.S. Department of Agriculture.

Stav Autorizace:

unapproved drug other

Charakteristika produktu

OSSAPIG-NF-NF1(NS) MINIATURE SWINE- NF1 HINDIII RESTRICTION FRAGMENT
LENGTH POLYMORPHISM (RFLP) POSITIVE FOR NF1-R1947X NF1(NS) ALLELE IN
OSSABAW
BREED SWINE NOT APPLICABLE
RECOMBINETICS, INC.
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OSSAPIG-NF-NF1(NS) MINIATURE SWINE
Identity: Transcription activator-like effector nuclease (TALEN) edit
and homology
dependent repair in Ossabaw breed porcine fetal fibroblasts to
introduce four base
substitution mutations into one copy of the NF1 gene of Sus scrofa
chromosome 12
(NC_010454.4). The C>T base substitution at base pair 43,530,892
generates a
premature stop codon in the coding sequence for the neurofibromin
protein and the
G>T base substitution at base pair 43,308,860 generates a HindIII
restriction enzyme
site used to genotype progeny from gene edited founders. The other two
substitutions,
C>T at 43,530,872 and T>C at 43,530,899, occur in the TALEN binding
sites and are
included to inhibit TALEN rebinding in the edited embryos.
Claim: Loss of NF1 gene function as a result of this gene edit in the
Ossabaw breed of
swine produces symptoms consistent with the National Institutes of
Health (NIH) criteria
for diagnosis of Neurofibromatosis Type I disease in humans.
Limitations: This is a model of progressive disease. Some of the
disease symptoms
associated with Neurofibromatosis Type I disease in humans take time
to develop, and
like the human disease, are variable in presentation from animal to
animal.
Product Use: Model animals and/or materials derived from these animals
are intended
for purchase only under license agreement with Recombinetics, Inc.,
and solely for use
in connection with the specific licensed biomedical research.
Conditions of Use: This model animal and any products derived from it
are for biomedical
research purposes only and not for use in humans. This is an
investigational animal.
Edible products of investigational animals are not to be used for food
unless
authorization has been granted by the U.S. Food and Drug
Administration or by the U.S.
Department of Agriculture.
WARNINGS