Реагенты, контроли, стандарты для биохимических исследований: реагенты:

Страна: Беларусь

Язык: русский

Источник: Министерством здравоохранения Республики (Центр экспертиз и испытаний в здравоохранении)

Доступна с:

Human Gesellschaft fur Biochemica und Diagnostica mbH, ГЕРМАНИЯ

класс:

изделия медицинского назначения

Производитель:

Human Gesellschaft fur Biochemica und Diagnostica mbH

Дата Авторизация:

2022-05-12

Характеристики продукта

Human Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
Telefon [phone] · Telefax [phone] · e-Mail
[e-mail]
A liquicolor
Photometric Colorimetric Test for Albumin
BCG-Method
Package Size
[REF]
10560
1 x 1000 ml
Complete test kit
156004
4 x 100 ml
Complete test kit
[IVD]
Method
1, 2
Bromocresol
green
forms
with
albumin
in
citrate
buffer
a
coloured
complex. The absorbance of this complex is proportional to the albumin
concentration in the sample.
Contents
[RGT]
4 x 100 or 1 x 1000 ml Colour reagent
Citrate buffer (pH 4.2)
30 mmol/l
Bromocresol green
260 µmol/l
[STD]
1 x 3 ml Standard
Albumin
4 g/dl or 40 g/l
Sodium azide
0.095 %
[STD]
is
standardised
against
the
certified
reference
material
CRM-
DA470k/IFCC.
Reagent Preparation
[RGT]
and
[STD]
are ready for use.
Reagent Stability
[RGT]
and
[STD]
are stable up to the given expiry date when stored at
2...25°C.
Contamination after opening must be avoided.
Specimen
Serum, heparinised or EDTA-plasma
Stability in serum:
at 2...8°C up to 1 month
at 15...25°C up to 1 week
Assay
Wavelength:
Hg 546 nm, 578 nm
Optical path:
1 cm
Temperature:
20...25°C
Measurement:
Against reagent blank. Only one reagent blank per series
is required.
Pipetting Scheme
Pipette into cuvettes
Reagent blank
Sample or
[STD]
Sample/
[STD]
[RGT]
---
1000 µl
10 µl
1000 µl
Mix, incubate for 5 min. at 20...25°C. Measure the absorbance of the
sample and the standard against the reagent blank within 30 min. (
∆
A).
Calculation of the Albumin Concentration
∆
A
sample
C = 4 x

[
g/dl]
∆
A
[STD]
or
∆
A
sample
C = 40 x

[
g/l]
∆
A
[STD]
Performance Characteristics
Linearity
The test is linear up to an albumin concentration of 6,5 g/dl or 65
g/l.
Dilute samples with a higher concentration 1 + 1 with physiological
saline
(0.9%). Multiply the result by 2.
Typical
performance
data
can
be
found
in
the
Verification
Report,
accessible via:
www.human.de/data/gb/vr/su-albu.p